Edaphocola flava sp. nov., Isolated from Herbicide Bensulfuron-methyl-Contaminated Soil.

A novel strain, HME-24T, was isolated from herbicide bensulfuron-methyl-contaminated soil in Jiangning District, Jiangsu Province, China. Strain HME-24T was a Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterium, and the colonies on R2A agar are yellow. The strain was non-sporulating, catalase- and oxidase-positive. Cells growth occurred at 15-37 °C (optimum, 30 °C), at pH 6.0-8.0 (optimum, 7.0) and with 0-1.5% (w/v) NaCl (optimum, 0.5% NaCl). Strain HME-24T showed the highest 16S rRNA gene sequence identity to Edaphocola aurantiacus H2T (99.58%), followed by Taibaiella chishuiensis AY17T (94.02%). The sole respiratory quinone was Menaquinone-7 (MK-7), the major polar lipids of strain HME-24T were two unidentified lipids, two unidentified aminolipids, an unidentified glycolipid and phosphatidylethanolamine, and the major fatty acids were iso-C15:0, iso-C15:1 G and iso-C17:0 3-OH. The genomic DNA G+C content values based on total genome sequences of strain HME-24T and H2T were 43.6 mol% and 43.8 mol%, respectively. Average nucleotide identity (ANI) for draft genomes between strain HME-24T and H2T was 94.8%, and the digital DNA-DNA hybridization (dDDH) for draft genomes between strain HME-24T and H2T was 59.2%. On the basis of phenotypic, genotypic and phylogenetic analysis, strain HME-24T represents a novel species of the genus Edaphocola, with the name Edaphocola flava sp. nov. being proposed. The type strain of Edaphocola flava is HME-24T (= KCTC 62977T = CGMCC 1.13935T).

Taibaiella helva sp. nov., isolated from farmland soil in China.

A yellow-coloured bacterial strain, designated F-4T, was isolated from a farmland soil sample from Qianshan, Anhui province, China. Strain F-4T was Gram-stain-negative, strictly aerobic, oval-shaped, motile (by gliding) and non-spore-forming. Growth occurred at 20-35 °C (optimum, 30 °C), at pH 6.0-8.0 (pH 7.0) and with 0-1.0 % (w/v) NaCl (0 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain F-4T belonged to the genus Taibaiella. 16S rRNA gene sequence similarity values between strain F-4T and the type strains of the three recognized species of the genus Taibaiella, Taibaiella koreensis KACC 17171T, Taibaiella soli KCTC 42277T and Taibaiella chishuiensis JCM 19637T, were 98.1, 96.4 and 95.9 %, respectively. The predominant respiratory quinone was MK-7, with MK-8 as a minor component. The major polar lipids of strain F-4T were three unidentified lipids, two unidentified aminolipids, three unidentified phospholipids, an unidentified aminophospholipid and phosphatidylethanolamine. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0. The G+C content of the genomic DNA based on total genome calculations was 51.3 mol%. The major polyamine of strain F-4T was homospermidine. The average nucleotide identity and the digital DNA-DNA hybridization values for draft genomes between strain F-4T and strain THG-DT86T were 79.8 and 22.6 %, respectively. On the basis of the genotypic and phenotypic data presented here, strain F-4T represents a novel species of the genus Taibaiella, for which the name Taibaiella helva sp. nov. is proposed. The type strain is F-4T (=KCTC 62442T=CGMCC 1.13562T).

Paenibacillus yanchengensis sp. nov., isolated from farmland soil.

A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3T, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China. Optimal growth occurred at 30 °C, pH 7.0-8.0 and 0.5 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3T was most closely related to Paenibacillusripae HZ1T (96.8 %), followed by Paenibacillussputi KIT00200-70066-1T (94.7 %). The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0. The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%. The major polar lipids of strain J-3T were aminolipid, phospholipid, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid and phosphatidylethanolamine. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. On the basis of genotypic and phenotypic data, strain J-3T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp. nov. is proposed. The type strain is J-3T (=KCTC 33926T=CGMCC 1.16455T).